Emicizumab (Genetical Recombination)



Emicizumab is a recombinant bispecific humanized monoclonal antibody composed of complementarity-determining regions derived from rat anti-human blood coagulation factor Ixa (FIXa) antibody and mouse anti-human blood coagulation factor X (FX) antibody, human framework regions and human IgG4 constant regions. In the anti-FIXa Hchain, the amino acid residues at position 202, 231, 299, 359, 412, 438 and 448 are substituted by Gln, Pro, Tyr, Lys,Lys, Arg and Pro, respectively, and Gly and Lys at the C-terminal are deleted. In the anti-FX H-chain, the amino acid residues at position 198, 227, 295, 408, 438 and 444 are substituted by Gln, Pro, Tyr, Lys, Glu and Pro, respectively, and Gly and Lys at the C-terminal are deleted. Emicizumab is produced in Chinese hamster ovary cells. Emicizumab is a glycoprotein (molecular weight: ca. 148,000) composed of an anti-FIXa H-chain (γ4-chain) consisting of 448 amino acid residues, an anti-FX H-chain (γ4-chain) consisting of 444 amino acid residues and 2 L-chains (κ-chains) consisting of 214 amino acid residues each.

[1610943-06-0]